Polyribosomes from Peas
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چکیده
منابع مشابه
Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors.
Profiles of polyribosomes were obtained from etiolated stem segments of Pisum sativum L. var. Alaska isolated in various buffers. Tissue homogenized in a medium containing 0.2 m tris-HCl, pH 8.5, 0.2 m sucrose, 30 mm MgCl(2), and 60 mm KCl yielded polyribosomes exhibiting far less degradation than tissue homogenized in conventional media containing tris-HCl at lower ionic strength and pH. A fur...
متن کاملPolyribosomes from Peas: VI. Auxin-stimulated Recruitment of Free Monosomes into Membrane-bound Polysomes.
Auxin treatment of aged pea stems (Pisum sativum L. var. Alaska) caused a decrease in monosomes (especially free monosomes) and an increase in polysomes (especially membrane-bound polysomes). These effects were not duplicated by gibberellic acid or benzyladenine. These auxin-stimulated shifts in polysome distribution commenced at least 9 hours before significant growth took place. It is suggest...
متن کاملPolyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population.
Attempts were made to isolate and characterize the total population of free and membrane-bound polysomes from the elongating region of dark-grown pea stems (Pisum sativum L.). Partial separation of free from membrane-bound polysomes was achieved by relatively low speed centrifugation of the homogenate. Complete separation was not achieved. Based on analysis of the rRNA content of various subcel...
متن کاملPolyribosomes from Peas AN IMPROVED METHOD FOR THEIR ISOLATION IN THE ABSENCE OF RIBONUCLEASE INHIBITORS ' Received for publication
Profiles of polyribosomes were obtained from etiolated stem segments of Pisum sativum L. var. Alaska isolated in various buffers. Tissue homogenized in a medium containing 0.2 M tris-HCI, pH 8.5, 0.2 M sucrose, 30 mM MgCL, and 60 mM KCI yielded polyribosomes exhibiting far less degradation than tissue homogenized in conventional media containing tris-HCl at lower ionic strength and pH. A furthe...
متن کاملPolyribosomes from Tetrahymena
The free-living, ciliated protist Tetrahymena, which can be grown in synchronized cultures, lends itself to studies of protein synthesis and organelle morphogenesis in eukaryotic cells . The isolation of polyribosomes, the sites of protein synthesis, from Tetrahymena was reported by Whitson et al . (5) . After lysis of the cells by indole-saturated buffer, the polysomes were isolated in 0 .01 M...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1976
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.57.4.516